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KMID : 0616120160470040366
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Korean Journal of Pharmacognosy
2016 Volume.47 No. 4 p.366 ~ p.373
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Development of an UPLC-DAD Method for Simultaneous Analysis of Eight Marker Compounds of Bulhwangeumjeonggi-san
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Lee Kyung-Hee
Ramakanta Lamichhane
Sharma Dipak Kumar
Pandeya Prakash Raj
Kim Se-Gun
Jung Hyun-Ju
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Abstract
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Bulhwangeumjeonggisan (BHGJGS) is a traditional herbal formulation generally used in the treatment of cold and gastritis. BHGJGS consists of eight herbal plants; Atractylodis Rhizoma, Magnoliae Cortex, Citri Pericarpium, Glycyrrhizae Radix, Agastachis Herba, Pinelliae Rhizoma, Zingiberis Rhizoma and Zizyphi Fructus. Complete standardization of this for-mulation has not been done yet. So, a simple and accurate method was developed and validated using Ultra Performance Liquid Chromatography (UPLC) with Diode Array Detector (DAD) for the standardization of BHGJGS. UPLC conditions were opti-mized using a c18 RP-Amide column with mobile phase; 0.1% phosphate buffer and acetonitrile, detection wavelength; 210 and 325 nm. The linearities of calibration curves were acceptable (R2 >0.9994), and the limit of detection and quantification were within the ranges of 0.011-0.091 and 0.034-0.277 μg/ml respectively. The relative standard deviation (RSD) of intra- and inter-day precisions were under 3.61%. The RSD of repeatability was under 0.68 %. The results of recovery test were 94.4- 107.9%, and the RSD were under 4.6%. The developed method was used to find the contents of standard constituents in BHG-JGS mix extract powder, and two commercial formulation (A and B). The data show that the developed method was specific, sensitive, accurate, and precise for analysis of BHGJGS components.
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KEYWORD
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Bulhwangeumjeonggisan, UPLC, Method validation, Simultaneous analysis of multiple components, Herbal formulation
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